A Rapid and Efficient Plant Regeneration Protocol using Leaves and Stems with Buds of Euphorbia pulcherrima Willd. ex Klotzsch (Euphorbiaceae)

Background: Euphorbia pulcherrima is highly ornamental value of poinsettia flower, which posses colorful bracts and low temperature resistant leave. Its embryos or adventitious buds were cultivated mainly from stems, petioles tender buds. The cultivation seedlings studied firstly for improving the quality Poinsettia. Methods: A rapid reproducible regeneration system leaves stems with has been established. There a comparative analysis results tissue culture different varieties Result: best medium component hormones about leaf callus inducing cluster formation comprise 6-BA 1.0 mg/L, KT 0.4 NAA 0.1 mg/L 2,4-D 0.05 mg/L. proliferation include 1.2 stem bud to germinate 1.5 addition level Ac have effect on length root quantity first second root.